The sawfishes (family Pristidae) are considered one of the most threatened groups of aquatic species, with all species assessed as Critically Endangered or Endangered on the IUCN Red List. The Largetooth Sawfish Pristis pristis was once globally widespread across the tropics but now northern Australia represents one of the species’ last remaining viable stocks (1). Nevertheless, this subpopulation has still undergone considerable declines in abundance and extent of its distribution. In northern Australia, many anthropogenic activities are considered threats to Largetooth Sawfish populations, including fishing and habitat modification (2). Effective management of the species is hampered by a lack of knowledge of the distribution, movement patterns, habitat use and requirements, life history and threats facings their populations. This information is required to provide a scientific basis for protection of critical habitats, mitigation of impacts, and regulation of harvest regimes (3). We aim to support the management of Largetooth Sawfish by providing an improved ecological understanding of juvenile Largetooth Sawfish. Acoustic tracking of juvenile Largetooth Sawfish in the Adelaide River of the Northern Territory is being undertaken using both active tracking and a passive receiver array. Preliminary results for the movement patterns and habitat use of Largetooth Sawfish are presented. Clearly identified and consistent movement patterns have been detected on larger spatial and temporal scales. On smaller spatial and temporal scales movement patterns are more variable.